Rapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols (Record no. 41835)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01619nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124702.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7507 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Rapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | FEMS MicroBiology Letters, 241(1), p.103, 2004 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We describe an allele specific PCR based approach for the rapid detection of two bovine Mycoplasma species associated with respiratory disease. Specific and universal oligonucleotides were used in combination to detect the presence of single nucleotide polymorphisms within the 16S ribosomal DNA sequence. Presence of Mycoplasma 16S rDNA is indicated by the production of a single control fragment, whilst positive samples generate an alternative smaller specific product over the same region. This technique provides a reliable and sensitive method which, although widely used in human genetic screening, has not been documented for diagnosis of bacterial infection. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MYCOPLASMA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DIAGNOSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IDENTIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ALLELE SPECIFIC POLYMERASE CHAIN REACTION (ASPCR) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SINGLE NUCLEOTIDE POLYMORPHISM (SNP) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MISMATCH |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Miles, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | McAuliffe, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayling, R.D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nicholas, R.A.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1WVwM3-W_XtlDcfSByqBbd5ABSLfiSE3_/view?usp=drivesdk">https://drive.google.com/file/d/1WVwM3-W_XtlDcfSByqBbd5ABSLfiSE3_/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7507 | 25.06.2025 | 25.06.2025 | Documentos solicitados |