CTAB-Urea Method Purifies RNA from Melanin for cDNA Microarray Analysis (Record no. 41863)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01594nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124703.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7535 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | CTAB-Urea Method Purifies RNA from Melanin for cDNA Microarray Analysis |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Pigment Cell Res, 17(3), p.312-315, 2004 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Melanin represents a major problem for the study of melanoma by microarrays since it is retained during RNA extraction and inhibits the enzymatic reactions used for probe preparation. Here we report a new method for cleaning RNA from melanin, based on the use of the cationic detergent cetyl-trimethylammonium bromide (CTAB)-urea for RNA precipitation. This method is easy to perform and has a low cost. Purified RNA is recovered with high quality and good yield. CTAB-urea treated RNA from highly pigmented melanoma cells can be successfully reverse transcribed and labeled to obtain probes which can be subsequently used in cDNA microarray experiments, giving consistent and reproducible results |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MELANOMA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MELANIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | METHOD |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RNA PURIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CDNA ARRAYS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lagonigro, M.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Cecco, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Carninci, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Di Stasi, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ranzani, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rodolfo, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gariboldi, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1Kjw27b1LfVQqOwfbmnFfgQPxMQH7C7u-/view?usp=drivesdk">https://drive.google.com/file/d/1Kjw27b1LfVQqOwfbmnFfgQPxMQH7C7u-/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7535 | 25.06.2025 | 25.06.2025 | Documentos solicitados |