Isolation of Nucleic Acids from Plants by Differential Solvent Precipitation (Record no. 41910)

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control field MX-MdCICY
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control field 20250625124704.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7582
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245 10 - TITLE STATEMENT
Title Isolation of Nucleic Acids from Plants by Differential Solvent Precipitation
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 195, p.45-50, 1991
520 3# - SUMMARY, ETC.
Summary, etc. The purification of nucleic acids from plant tissue is often made difficult by the presence of contaminating carbohydrate polymers and polyphenols. A procedure for the simultaneous isolation of DNA and translatable RNA from plants is described. The method removes most of the polysaccharides and polyphenols extracted with nucleic acids in a single step by taking advantage of differences in solubility of these compounds in the solvent 2-butoxyethanol. Stepwise addition of 2-butoxyethanol to phenol extracts of specific ionic strength precipitates nucleic acids largely free of contaminants. Subsequent separation of RNA from DNA by precipitation with LiCl was optimised to give a high recovery of translationally active RNA. Successful isolation of nucleic acids from strawberry (Fmgaria X ananassa)receptacle, a particularly recalcitrant tissue, and from a range of tissues of other plant species demonstrates the eneral applicability of the method.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Manning, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1lUpOM2p694oTOuE_bPe_nHt1NeMh-xly/view?usp=drivesdk">https://drive.google.com/file/d/1lUpOM2p694oTOuE_bPe_nHt1NeMh-xly/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7582 25.06.2025 25.06.2025 Documentos solicitados