Identification of a lipopolysaccharide responsive erk-like MAP kinase in tobacco leaf tissue (Record no. 41934)
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| fixed length control field | 02310nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124704.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7610 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Identification of a lipopolysaccharide responsive erk-like MAP kinase in tobacco leaf tissue |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Plant Pathology, 5(4), p.331-342, 2004 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Lipopolysaccharides (LPS)are indispensable cell surface components of Gram-negative bacteria and have diverse roles in plant-microbe interactions. Treatment of Nicotiana tabacum with the LPS of an endophytic strain of Burkholderia cepacia results in an enhanced defensive capacity in the tissue. In this study the rapid and transient phosphorylation of an extracellular signal-regulated (ERK)-like mitogen-activated protein (MAP)kinase in response to LPS from B. cepacia (LPS B.cep. ) elicitation is reported. Based on in-gel kinase assays it was found that this 43-kDa LPS B.cep. -responsive kinase is optimally activated following 7 min elicitation with 100 ì g/mL LPS. Its identity as an ERK MAPK was supported by tyrosinephosphorylated association with induction, immunodetection with pTEpY-specific MAPK antibodies and inhibition of phosphorylation by U0126, an upstream MAPKK inhibitor. The kinase utilized myelin basic protein, but not casein or histone, as substrate. Ca 2+ did not modulate the phosphorylation, nor did wounding. To date, other MAP kinases have been shown to act either independently or upstream from reactive oxygen intermediates produced during the oxidative burst. It was found that hydrogen peroxide is either not generated in leaf tissue in response to LPS elicitation or, if generated, does not trigger the phosphorylation of the kinase. Physicochemical characterization of the ERK-like MAPK indicated a molecular mass of 43 kDa and a pI of 6.3; two-dimensional gel analysis indicated two charge isomers. This is the first demonstration of such an LPS-responsive MAP kinase phosphorylation in plants. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Piater, L.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nürnberger, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dubery, I.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1WYoD-mjLjEhA3dPZmGeFjCG0TmJiKyXQ/view?usp=drivesdk">https://drive.google.com/file/d/1WYoD-mjLjEhA3dPZmGeFjCG0TmJiKyXQ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7610 | 25.06.2025 | 25.06.2025 | Documentos solicitados |