Molecular Basis for the RIN4 Negative Regulation of RPS2 Disease Resistance (Record no. 41954)

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control field MX-MdCICY
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control field 20250625124704.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7630
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Title Molecular Basis for the RIN4 Negative Regulation of RPS2 Disease Resistance
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell, 17, p.1292-1305, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Recent studies have demonstrated that RPS2, a plasma membrane-localized nucleotide binding site/leucine-rich repeat protein from Arabidopsis thaliana, associates with RPM1 Interacting Protein 4 (RIN4)and that this association functions to modulate the RPS2-mediated defense pathway in response to the bacterial effector protein AvrRpt2. In addition to negatively regulating RPS2 activity, RIN4 is also a target of AvrRpt2, a Cys protease and cognate bacterial effector protein of RPS2. Nicotiana benthamiana has been employed as a heterologous xpression system to characterize the RPS2-RIN4 association, defining the domains in RIN4 required for the negative regulation of RPS2 activity. Upon inoculation of N. benthamiana leaves with Agrobacterium tumefaciens expressing RPS2, a rapid hypersensitive response (HR)is detected with 22 h of infiltration. The HR can be blocked by infiltrating the leaf with A. tumefaciens expressing RPS2 in the presence of RIN4, recapitulating the ability of RIN4 to interfere with RPS2-mediated resistance in Arabidopsis. Moreover, in the presence of RIN4, the RPS2-mediated HR can be restored by the delivery of AvrRpt2 via A. tumefaciens. This assay has been developed as a phenotypic marker for (1)the HR-inducing phenotype associated with RPS2, (2)negative regulation of RPS2 by RIN4, and (3)the AvrRpt2-mediated disappearance of RIN4. Here, we present a series of deletion and site-directed mutation analyses to identify amino acids in RIN4 required for the RPS2-RIN4 association and to distinguish these from residues in RIN4 that serve as a target sequence for AvrRpt2. In addition to characterizing the RPS2-RIN4 association in N. benthamiana, we have moved forward to show that the biological relevance of these amino acid changes is applicable in Arabidopsis as well. To this end, we have identified specific amino acids within the C-terminal half of RIN4 that are required for RPS2 regulation and association.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Day, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dahlbeck, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Huang, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chisholm, S.T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Staskawicz, B.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1VbY1ghjCVvF8GlaHKLmmDISclL5MAzkb/view?usp=drivesdk">https://drive.google.com/file/d/1VbY1ghjCVvF8GlaHKLmmDISclL5MAzkb/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7630 25.06.2025 25.06.2025 Documentos solicitados