Identification of a Protein from Rust Fungi Transferred from Haustoria into Infected Plant Cells (Record no. 41977)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02586nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124705.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7653 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Identification of a Protein from Rust Fungi Transferred from Haustoria into Infected Plant Cells |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Plant-Microbe Interactions, 18, p.1130-1139, 2005 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The formation of haustoria is one of the hallmarks of the interaction of obligate biotrophic fungi with their host plants. In addition to their role in nutrient uptake, it is hypothesized that haustoria are actively involved in establishing and maintaining the biotrophic relationship. We have identified a 24.3-kDa protein that exhibited a very unusual allocation. Rust transferred protein 1 from Uromyces fabae (Uf-RTP1p)was not only detected in the host parasite interface, the extrahaustorial matrix, but also inside infected plant cells by immunofluorescence and electron microscopy. Uf-RTP1p does not exhibit any similarity to sequences currently listed in the public databases. However, we identified a homolog of Uf-RTP1p in the related rust fungus Uromyces striatus (Us-RTP1p). The localization of Uf-RTP1p and Us-RTP1p inside infected plant cells was confirmed, using four independently raised polyclonal antibodies. Depending on the developmental stage of haustoria, Uf-RTP1p was found in increasing amounts in host cells, including the host nucleus. Putative nuclear localization signals (NLS)were found in the predicted RTP1p sequences. However, functional efficiency could only be verified for the Uf-RTP1p NLS by means of green fluorescent protein fusions in transformed tobacco protoplasts. Western blot analysis indicated that Uf-RTP1p and Us-RTP1p most likely enter the host cell as N-glycosylated proteins. However, the mechanism by which they cross the extrahaustorial membrane and accumulate in the host cytoplasm is unknown. The localization of RTP1p suggests that it might play an important role in the maintenance of the biotrophic interaction. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HAUSTORIUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MEDICAGO TRUNCATULA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEIN SECRETION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | VICIA FABA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kemen, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kemen, A.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rafiqi, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hempel, U. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mendgen, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hahn, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Voegele, R.T. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1GITc9LJaNGx1J8T8e0xI-y0oIPYfhi2z/view?usp=drivesdk">https://drive.google.com/file/d/1GITc9LJaNGx1J8T8e0xI-y0oIPYfhi2z/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7653 | 25.06.2025 | 25.06.2025 | Documentos solicitados |