Identification and Characterization of a Stress-Inducible and a Constitutive Small Heat-Shock Protein Targeted to the Matrix of Plant Peroxisomes1[W] (Record no. 42067)

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control field 20250625124707.0
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7745
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Title Identification and Characterization of a Stress-Inducible and a Constitutive Small Heat-Shock Protein Targeted to the Matrix of Plant Peroxisomes1[W]
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Volume/sequential designation Plant Physiology, 141, p.47-60, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Small heat-shock proteins (sHsps)are widespread molecular chaperones for which a peroxisomal localization has not yet been reported. The Arabidopsis (Arabidopsis thaliana)genome encodes two sHsps with putative peroxisomal targeting signals type 1 or 2 (PTS1 or PTS2). As demonstrated by double-labeling experiments using full-length fusion proteins with enhanced yellow fluorescent protein and deletion constructs lacking the putative targeting domains, AtHsp15.7 (At5g37670)and AtAcd31.2 (At1g06460)are targeted to the peroxisome matrix by a functional PTS1 (SKL.)and a functional PTS2 (RLx5HF), respectively. The peroxisomal localization of AtAcd31.2 was further confirmed by isolation of leaf peroxisomes from Arabidopsis by two successive sucrose density gradients, protein separation by one- and two-dimensional gel lectrophoresis, and mass spectrometric protein identification.When AtHsp15.7 and AtAcd31.2 were heterologously expressed in yeast (Saccharomyces cerevisiae)and directed to the cytosol by deletion of the PTSs, both sHsps were able to complement the morphological phenotype of yeast mutants deficient in the cytosolic homologs ScHsp42 or ScHsp26. According to expression studies by reverse transcription-PCR, AtAcd31.2 is constitutively expressed, whereas AtHsp15.7 is hardly expressed under normal conditions but strongly induced by heat and oxidative stress, the latter of which was triggered by the catalase inhibitor 3-aminotriazole or the herbicide methyl viologen applied by watering of whole plants or infiltration of rosette leaves. Thus, plants are exceptional among eukaryotes in employing sHsps in the peroxisome matrix to prevent unspecific aggregation of partially denatured proteins under both physiological and stress conditions.
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Personal name Ma, Ch.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Haslbeck, M.
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Personal name Babujee, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jahn, O.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Reumann, S.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1eDoNWWt7w0ipLPJF20w47EIz5PQ7_Clk/view?usp=drivesdk">https://drive.google.com/file/d/1eDoNWWt7w0ipLPJF20w47EIz5PQ7_Clk/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7745 25.06.2025 25.06.2025 Documentos solicitados