Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley (Record no. 42070)
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| fixed length control field | 02583nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124707.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7748 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Physiology, 138, p.2155-2164, 2005 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We successfully implemented virus-induced gene silencing (VIGS)in barley (Hordeum vulgare)for the functional characterization of genes required for Mla13-mediated resistance toward the biotrophic barley pathogen Blumeria graminis f. sp. hordei. Initially, barley cultivars were screened for their ability to host the barley stripe mosaic virus (BSMV)-VIGS vector by allowing its replication and systemic movement without causing excessive symptoms. Phytoene desaturase silencing leading to photobleaching was used as a phenotypic marker alongside reverse transcription-PCR data to characterize the silencing response at the molecular level. Barley cultivar Clansman, harboring the Mla13 resistance gene, was chosen as the most suitable host for BSMV-VIGS-based functional characterization of Rar1, Sgt1, and Hsp90 in the Mla-mediated resistance toward powdery mildew.BSMV-induced gene silencing of these candidate genes, which are associated in many but not all race-specific pathways, proved to be robust and could be detected at bothmRNAand protein levels for up to 21 d postinoculation. Systemic silencing was observed not only in the newly developed leaves from the main stem but also in axillary shoots. By examining fungal development from an incompatible mildew strain carrying the cognate Avr13 gene on plants BSMV silenced for Rar1, Sgt1, and Hsp90, a resistance-breaking phenotype was observed, while plants infected with BSMV control constructs remained resistant. We demonstrate that Hsp90 is a required component for Mla13-mediated race-specific resistance and that BSMV-induced VIGS is a powerful tool to characterize genes involved in pathogen resistance in barley. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hein, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Barciszewska-Pacak, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hrubikova, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Williamson, |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dinesen, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soenderby, I.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sundar, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sundar, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shirasu, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lacomme, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/15N33-LQOtGhUHZG9vmLXNRiIzPtOyCe2/view?usp=drivesdk">https://drive.google.com/file/d/15N33-LQOtGhUHZG9vmLXNRiIzPtOyCe2/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7748 | 25.06.2025 | 25.06.2025 | Documentos solicitados |