Determination of nucleic acids based on the fluorescence quenching of Hoechst 33258 at pH 4.5 (Record no. 42143)
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| 000 -LEADER | |
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| fixed length control field | 02068nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124708.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7830 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Determination of nucleic acids based on the fluorescence quenching of Hoechst 33258 at pH 4.5 |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytica Chimica Acta, 570(1), p.21-28, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | It was found the strong fluorescence emitted by the bis-benzimidazole derivative Hoechst 33258 at 490 nm could be efficiently quenched in pH 4.5 buffer when nucleic acids were added. Analysis of fluorescence intensity showed that the procedure was a static quenching dominated one, which was also demonstrated by the electron absorption spectra and lifetime of the excited state. The binding constant and numbers of binding sites were obtained from the Scatchard plot. The decreased fluorescence intensity was in proportion to the concentration of nucleic acids in the range 40-1800 ng ml-1 for dsDNA and 26-1700 ng ml-1 for ssDNA. The limits of detection were 12 and 8 ngml-1, respectively. The sensitivity of the method was about 3.4 times higher for dsDNA detection and 5.4 times higher for ssDNA detection compared with the widely used fluorescence enhancement method using the same dye. Application results to synthetic samples showed simplicity, rapidity and satisfactory reproducibility of the presented method. Measurement of real samples extracted from leaves of Crassula argentea and E. coli genome also gave satisfactory results, which were in good agreement with those obtained using spectrophotometric method. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | QUANTITATIVE DETECTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NUCLEIC ACIDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HOECHST 33258 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FLUORESCENCE QUENCHING |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guan, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhou, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yao, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1V2wv8fqqj5NGW-UTz5YRWJKDWteFNpEI/view?usp=drivesdk">https://drive.google.com/file/d/1V2wv8fqqj5NGW-UTz5YRWJKDWteFNpEI/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7830 | 25.06.2025 | 25.06.2025 | Documentos solicitados |