The Ga Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica (Record no. 42209)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01952nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124709.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7896 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | The Ga Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | The Plant Cell, 18, p.1510-1523, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The function of a Ga protein in the elicitation of phytoalexin (benzophenanthridine)biosynthesis was characterized in cultured cells of California poppy (Eschscholzia californica). Both the decrease of Ga content via antisense transformation and the expression of recombinant anti-Ga single-chain antibodies strongly impaired the induction of alkaloid biosynthesis by low elicitor concentrations. All transgenic cell types were deficient in two elicitor-triggered early signal events: activation of phospholipase A2 (PLA2)and efflux of vacuolar protons. The lacking Hþ efflux could be restored (1)by adding lysophosphatidylcholine (LPC), a product of PLA2 activity, to vacuoles in situ and (2)by exposing intact cells to isotonic, near-neutral HEPES buffers. The latter treatment induced alkaloid biosynthesis in the absence of elicitor and in Ga-deficient cells. We conclude that Ga mediates the stimulation of PLA2 by low elicitor concentrations and that the resulting peak of LPC initiates a transient efflux of vacuolar protons. In this way, an acidic peak of the cytoplasmic pH is generated that causes the expression of enzymes of phytoalexin production independent of the hypersensitive response. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Viehweger, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schwartze, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schumann, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lein, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Roosa, W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1gADwJ-E06LcU0zjZepG7q-wYFTRYY1fB/view?usp=drivesdk">https://drive.google.com/file/d/1gADwJ-E06LcU0zjZepG7q-wYFTRYY1fB/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7896 | 25.06.2025 | 25.06.2025 | Documentos solicitados |