MARC details
| 000 -LEADER |
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| fixed length control field |
02772nam a2200373Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625124711.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-7952 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Proteome analysis of hairy root from Panax ginseng C. A. Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Proteomics, 3(12), p.2379-2392, 2003 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
As an initial step to the comprehensive proteomic analysis of Panax ginseng C. A. Meyer, protein mixtures extracted from the cultured hairy root of Panax ginseng were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). The protein spots were analyzed and identified by peptide finger printing and internal amino acid sequencing by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)and electrospray ionization quadrupole-time of flight mass spectrometry (ESI Q-TOF MS), respectively. More than 300 protein spots were detected on silver stained two-dimensional (2-D)gels using pH 3-10, 4-7, and 4.5- 5.5 gradients. Major protein spots (159)were analyzed by peptide fingerprinting or de novo sequencing and the functions of 91 of these proteins were identified. Protein identification was achieved using the expressed sequence tag (EST)database from Panax ginseng and the protein database of plants like Arabidopsis thaliana and Oryza sativa. However, peptide mass fingerprinting by MALDI-TOF MS alone was insufficient for protein identification because of the lack of a genome database for Panax ginseng. Only 17 of the 159 protein spots were verified by peptide mass fingerprinting using MALDI-TOF MS whereas 87 out of 102 protein spots, which included 13 of the 17 proteins identified by MALDI-TOF MS, were identified by internal amino acid sequencing using tandem mass spectrometry analysis by ESI Q-TOF MS. W ten the internal amino acid sequences were used as identification markers, the identification rate exceeded 85.3 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EXPRESSED SEQUENCE TAG DATA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HAIRY ROOT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
INTERNAL SEQUENCING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PANAX GINSENG |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
C. A. MEYER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PEPTIDE FINGERPRINTING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTEOME ANALYSIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kim, S.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jin Young Kim |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Eun A. Kim |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kyung-Hoon Kwon |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kyung-Wook Kim |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kun Cho |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jeong Hwa Lee |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jeong Hwa Lee |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Deok-Chun Yang |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jong Shin Yoo |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Young Mok Park |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/18GrJZJQcEgGB-3t0M-XrdbnITUl8NVgZ/view?usp=drivesdk">https://drive.google.com/file/d/18GrJZJQcEgGB-3t0M-XrdbnITUl8NVgZ/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
