MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02850nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625124711.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-7971 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Plant Journal, 47(4), p.547-563, 2006 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
The benzylisoquinoline alkaloids of opium poppy, including the narcotic analgesics morphine and codeine, accumulate in the multinucleate cytoplasm of specialized laticifers that accompany vascular tissues throughout the plant. In mature opium poppy plants, immunofluorescence labeling using specific antibodies showed that four alkaloid biosynthetic enzymes, (S)-norcoclaurine 6-O-methyltransferase (6OMT), (S)-coclaurine N-methyltransferase (CNMT), (S)-3¢-hydroxy-N-methylcoclaurine-4¢-O-methyltransferase (4¢OMT)and alutaridinol-7-O-acetyltransferase (SAT)were restricted to sieve elements of the phloem adjacent or proximal to laticifers. The identity of sieve elements was confirmed by (i)the specific immunogold labeling of the characteristic cytoplasm of this cell type, (ii)the co-localization of a sieve element-specific Hþ-ATPase with all biosynthetic enzymes and (iii)the strict association of sieve plates with immunofluorescent cells. The localization of laticifers was demonstrated antibodies specific to major latex protein (MLP), which is characteristic of this cell type. In situ hybridization using antisense RNA probes for 6OMT, CNMT, 4¢OMT and SAT showed that the corresponding gene transcripts were found in the companion cell paired with each sieve element. Seven benzylisoquinoline alkaloid biosynthetic enzymes, (S)-N-methylcoclaurine 3¢-hydroxylase (CYP80B1), berberine bridge enzyme, codeinone reductase, 6OMT, CNMT, 4¢OMT and SAT were localized by immunofluorescence labeling to the sieve elements in the root and hypocotyl of opium poppy seedlings. The abundance of these enzymes increased rapidly between 1 and 3 days after seed germination. The localization of seven biosynthetic enzymes to the sieve elements provides strong support for the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
BENZYLISOQUINOLINE ALKALOIDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
IMMUNOFLUORESCENCE LABELING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
IMMUNOGOLD LABELING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
IN SITU HYBRIDIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PAPAVER SOMNIFERUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SECONDARY METABOLISM |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Samanani, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Alcantara, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bourgault, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zulak, K.G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Facchini, P.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1nkwiaTs-gcUrVa_wgVOes28_aBXL9h8i/view?usp=drivesdk">https://drive.google.com/file/d/1nkwiaTs-gcUrVa_wgVOes28_aBXL9h8i/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
