Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis (Record no. 42294)
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| fixed length control field | 01818nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124711.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-7982 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Virological Methods, 135(2), p.288-291, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Marek's disease virus (MDV)is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in poultry. The MDV genome encodes more than 100 genes. However, the role of many of these genes in virus replication is not known. The construction of an infectious bacterial artificial chromosome (BAC)clone of the highly oncogenic RB-1B strain ofMDV has been described previously. Virus reconstituted from the BAC clone induced rapid-onset lymphomas in chickens very similar to the wildtype viruses. In this paper, the construction of a high-density random transposon-insertion mutant library of the RB-1B BAC clone using a high throughput in vitro transposon mutagenesis technique is described. Furthermore a PCR screening method, using primers specific for the transposon sequence and the MDV gene(s)of interest, was developed for the rapid identification of specific insertion mutants. The application of the screening method to identify some of the non-essential genes for MDV replication in vitro is described. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MDV |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BAC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSPOSON MUTAGENESIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chattoo, J.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Stevens, M.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nair, V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/17FW5t-TAOVFULME82eLGXfnFPiRJ9sQ2/view?usp=drivesdk">https://drive.google.com/file/d/17FW5t-TAOVFULME82eLGXfnFPiRJ9sQ2/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-7982 | 25.06.2025 | 25.06.2025 | Documentos solicitados |