Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera (Record no. 42306)

MARC details
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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
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control field 20250625124711.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-7994
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Title Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera
490 0# - SERIES STATEMENT
Volume/sequential designation FEMS Yeast Research, 1(4), p.279-289, 2002
520 3# - SUMMARY, ETC.
Summary, etc. Three molecular methods, RAPD-PCR analysis, electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions (ITS1, ITS2 and the intervening 5.8S rDNA), were studied for accurate identification of Hanseniaspora and Kloeckera species as well as for determining inter- and intraspecific relationships of 74 strains isolated from different sources and/or geographically distinct regions. Of these three methods, PCR-RFLP analysis of ITS regions with restriction enzymes DdeI and HinfI is proposed as a rapid identification method to discriminate unambiguously between all six Hanseniaspora species and the single non-ascospore-forming apiculate yeast species Kloeckera lindneri. Electrophoretic karyotyping produced chromosomal profiles by which the seven species could be divided into four groups sharing similar karyotypes. Although most of the 60 strains examined exhibited a common species-specific pattern, a different degree of chromosomal-length polymorphism and a variable number of chromosomal DNA fragments were observed within species. Cluster analysis of the combined RAPD-PCR fingerprints obtained with one 10-mer primer, two microsatellite primers and one minisatellite primer generated clusters which with a few exceptions are in agreement with the groups as earlier recognized in DNA^DNA homology studies
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element APICULATE YEAST
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IDENTIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR-RFLP ANALYSIS OF RDNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELECTROPHORETIC KARYOTYPING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RAPD-PCR ANALYSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FINGERPRINTING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cadez, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Raspor, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name de Cock, A.W.A.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Boekhout, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Smith, M.T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1b9xpDwlW1RafEnCh2Vf8QIZa5d8E3bWD/view?usp=drivesdk">https://drive.google.com/file/d/1b9xpDwlW1RafEnCh2Vf8QIZa5d8E3bWD/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-7994 25.06.2025 25.06.2025 Documentos solicitados