Efficient micropropagation of Robinia ambigua var. idahoensis (Idaho Locust)and detection of genomic variation by ISSR markers (Record no. 42339)

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control field 20250625124712.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8028
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Title Efficient micropropagation of Robinia ambigua var. idahoensis (Idaho Locust)and detection of genomic variation by ISSR markers
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Volume/sequential designation Plant Cell, Tissue and Organ Culture , 84(3), p.343-351, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Robinia ambigua var. idahoensis, presumably originated from interspecific hybridization of R. pseudoacacia L. and R. hispida L., is a multipurpose tree. Several reports have showed that in vitro micropropagation is a feasible method to produce large quantities of 'clonal' plants from R. pseudoacacia, however, no information is available on micropropagation of R. ambigua or the other assumed parental species, R. hispida. Here, we report on a tissue culture system for efficient micropropagation of R. ambigua plants by enhanced branching of axillary buds taken from a single branch of a donor tree. The culture system consists of sequential use of three media, namely, the bud-induction medium (MS medium supplemented with 0.8-1.4 mg l)1 6-BA, 0.05-0.08 mg l)1 NAA and 0.07-0.1 mg l)1 GA), elongation medium (MS medium added with 0.35-0.5 mg l)1 6-BA, 0.05-0.08 mg l)1 NAA and 0.07-0.1 mg l)1 GA)and root-induction medium (1/4 MS medium fortified with 1.7-2.5 mg l)1 IAA and 0.1-0.5 mg l)1 IBA). In addition, we investigated the genetic stability (relative to the donor plant)of a sample of 41 morphologically normal plants randomly taken from ca. 13,000 micropropagated plants, by using the intersimple sequence repeat (ISSR)marker with 32 selected primers. We found that of the 226 reproducible bands scored, 24 were polymorphic (10.62
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guo, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gong, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dong, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1iVb2pedh0kQVERNtnD58K4neQKAX7XvZ/view?usp=drivesdk">https://drive.google.com/file/d/1iVb2pedh0kQVERNtnD58K4neQKAX7XvZ/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8028 25.06.2025 25.06.2025 Documentos solicitados