Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades (Record no. 42361)

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control field 20250625124713.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8050
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Title Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades
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Volume/sequential designation The Plant Journal, 44(2), p.314-333, 2005
520 3# - SUMMARY, ETC.
Summary, etc. work has revealed that the cytokinin signal is transduced by two-component systems to the nucleus where target genes are activated. Most of the rapid transcriptional responses are unknown. We measured immediateearly and delayed cytokinin responses through genome-wide expression profiling with the Affymetrix ATH1 full genome array (Affymetrix Inc., Santa Clara, CA, USA). Fifteen minutes after cytokinin treatment of 5-dayold Arabidopsis seedlings, 71 genes were upregulated and 11 genes were downregulated. Immediate-early cytokinin response genes include a high portion of transcriptional regulators, among them six transcription factors that had previously not been linked to cytokinin. Five plastid transcripts were rapidly regulated as well, indicating a rapid transfer of the signal to plastids or direct perception of the cytokinin signal by plastids. After 2 h of cytokinin treatment genes coding for transcriptional regulators, signaling proteins, developmental and hormonal regulators, primary and secondary metabolism, energy generation and stress reactions were overrepresented. A significant number of the responding genes are known to regulate light (PHYA, PSK1, CIP8, PAT1, APRR), auxin (Aux/IAA), ethylene (ETR2, EIN3, ERFs/EREBPs), gibberellin (GAI, RGA1, GA20 oxidase), nitrate (NTR2, NIA)and sugar (STP1, SUS1)dependent processes, indicating intense crosstalk with environmental cues, other hormones and metabolites. Analysis of cytokinin-deficient 35S:AtCKX1 transgenic seedlings has revealed additional, long-lasting cytokinin-sensitive changes of transcript abundance. Comparative overlay-analysis with the software tool MAPMAN identified previously unknown cytokinin-sensitive metabolic genes, for example in the metabolism of trehalose-6-phosphate. Taken together, we present a genome-wide view of changes in ytokinin-responsive transcript abundance of genes that might be functionally relevant for the many biological processes that are governed by cytokinins.
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Topical term or geographic name entry element CYTOKININ
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENE EXPRESSION
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Topical term or geographic name entry element IMMEDIATE-EARLY GENES
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Topical term or geographic name entry element RESPONSE GENES
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Topical term or geographic name entry element TRANSCRIPTOME ANALYSIS.
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Personal name Brenner, W.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Romanov, G.A.
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Personal name Kollmer, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Burkle, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schmulling, T.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1i45E3ugsWyQWqUBtzBd6ZSlhFM8GtYnl/view?usp=drivesdk">https://drive.google.com/file/d/1i45E3ugsWyQWqUBtzBd6ZSlhFM8GtYnl/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8050 25.06.2025 25.06.2025 Documentos solicitados