Trehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation (Record no. 42385)

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fixed length control field 02079nam a2200313Ia 4500
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control field MX-MdCICY
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control field 20250625124713.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8076
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245 10 - TITLE STATEMENT
Title Trehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Biotechnology, 121(3), p.309-317, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Insertion of foreign DNA into plant genomes occurs randomly and with low frequency. Hence, a selectable marker is generally required to identify transgenic plants. Until now, all selection systems have been based on the use of non-plant genes, derived from microorganisms and usually conferring antibiotic or herbicide resistance. The use of microorganism-derived genes however has raised biosafety concerns.We have developed a novel selection system based on enhancing the expression of a plant-intrinsic gene and the use of a harmless selection agent. Selection takes advantage of the reduced glucose sensitivity of seedlings with enhanced expression of AtTPS1, a gene encoding trehalose-6-P synthase. As a result, transformants can be identified as developing green seedlings amongst the background of small, pale non-transformed plantlets on high glucose medium. In addition, vegetative regeneration of tobacco leaf explants is very sensitive to high external glucose. Overexpression of AtTPS1 in tobacco allows selecting glucose insensitive transgenic shoots.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INTRINSIC SELECTION MARKER
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Topical term or geographic name entry element ATTPS1
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TREHALOSE-6-PHOSPHATE SYNTHASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUCOSE RESISTANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS THALIANA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NICOTIANA TABACUM
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leymana, B.
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Personal name Avonce, N.
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Personal name Ramona, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Van Dijck, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Iturriaga, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Thevelein, J.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1BbjSohvbIc-4mn63isCf7YsjGsr30uv-/view?usp=drivesdk">https://drive.google.com/file/d/1BbjSohvbIc-4mn63isCf7YsjGsr30uv-/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8076 25.06.2025 25.06.2025 Documentos solicitados