Biochemical Characterization of the First Essential Two-Component Signal Transduction System from Staphylococcus aureus and Streptococcus pneumoniae (Record no. 42425)
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| fixed length control field | 02440nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124714.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8116 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Biochemical Characterization of the First Essential Two-Component Signal Transduction System from Staphylococcus aureus and Streptococcus pneumoniae |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Molecular MicroBiology and Biotechnology, 5(4), p.252-260, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The yycFG two-component signal transduction system (TCSTS)has been shown to be essential to the viability of several gram-positive bacteria. However, the function of the gene pair remains unknown. Interestingly, while both components are essential to Staphylococcus aureus and Bacillus subtilis, only the response regulator (yycF)is essential to Streptococcus pneumoniae. To study this essential TCSTS further, the S. pneumoniae and S. aureus truncated YycG histidine kinase and full-length YycF response regulator proteins were characterized at a biochemical level. The recombinant proteins from both organisms were expressed in Escherichia coli and purified. The YycG autophosphorylation activities were activated by ammonium. The apparent Km (ATP)of S. aureus YycG autophosphorylation was 130 ÌM and S. pneumoniae was 3.0 ÌM. Each had similar kcat values of 0.036 and 0.024 min-1, respectively. Cognate phosphotransfer was also investigated indicating different levels of the phosphorylated YycG intermediates during the reaction. The S. pneumoniae YycG phosphorylated intermediate was not detectable in the presence of its cognate YycF, while phosphorylated S. aureus YycG and YycF were detected concurrently. In addition, noncognate hosphotransfer was demonstrated between the two species. These studies thoroughly compare the essential YycFG TCSTS from the two species at the biochemical level and also establish methods for assaying the activities of these antibacterial targets. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | YYCF |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | YYCG |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RESPONSE REGULATOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HISTIDINE KINASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Clausen, V.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bae, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Throup, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Burnham, M.K.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rosenberg, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wallis, N.G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1A0vDO__ht5JrZLCwAmwza9j7mZ33_NJu/view?usp=drivesdk">https://drive.google.com/file/d/1A0vDO__ht5JrZLCwAmwza9j7mZ33_NJu/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8116 | 25.06.2025 | 25.06.2025 | Documentos solicitados |