Visualizing Plant Development and Gene Expression in Three Dimensions Using Optical Projection Tomography (Record no. 42441)

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control field 20250625124714.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8133
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Title Visualizing Plant Development and Gene Expression in Three Dimensions Using Optical Projection Tomography
490 0# - SERIES STATEMENT
Volume/sequential designation The Plant Cell, 18(9), p.2145-2156, 2006
520 3# - SUMMARY, ETC.
Summary, etc. A deeper understanding of the mechanisms that underlie plant growth and development requires quantitative data on threedimensional (3D)morphology and gene activity at a variety of stages and scales. To address this, we have explored the use of optical projection tomography (OPT)as a method for capturing 3D data from plant specimens. We show that OPT can be conveniently applied to a wide variety of plant material at a range of scales, including seedlings, leaves, flowers, roots, seeds, embryos, and meristems. At the highest resolution, large individual cells can be seen in the context of the surrounding plant structure. For naturally semitransparent structures, such as roots, live 3D imaging using OPT is also possible. 3D domains of gene expression can be visualized using either marker genes, such as b-glucuronidase, or more directly by whole-mount in situ hybridization. We also describe tools and software that allow the 3D data to be readily quantified and visualized interactively in different ways.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, K.
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Personal name Avondo, J.
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Personal name Morrison, H.
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Personal name Blot, L.
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Personal name Stark, M.
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Personal name Sharpe, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bangham, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bangham, A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xYnKdARjnYXgz2n1Qsbqe-txniEU4lqq/view?usp=drivesdk">https://drive.google.com/file/d/1xYnKdARjnYXgz2n1Qsbqe-txniEU4lqq/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8133 25.06.2025 25.06.2025 Documentos solicitados