The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen (Record no. 42462)

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fixed length control field 02009nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124715.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8155
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen
490 0# - SERIES STATEMENT
Volume/sequential designation J Biol Inorg Chem, 9(5), p.616-626, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Infrared spectra of 15N-enriched preparations of the soluble cytoplasmic NAD+-reducing [NiFe]- hydrogenase from Ralstonia eutropha are resented. These spectra, together with chemical analyses, show that the Ni-Fe active site contains four cyanide groups and one carbon monoxide molecule. It is proposed that the active site is a (RS)2(CN)Ni(l-RS)2Fe(CN)3(CO)centre (R=Cys)and that H2 activation solely takes place on nickel. One of the two FMN groups (FMN-a)in the enzyme can be reversibly released upon reduction of the enzyme. It is now reported that at longer times also one of the cyanide groups, the one proposed to be bound to the nickel atom, could be removed from the enzyme. This process was irreversible and induced the inhibition of the enzyme activity by oxygen; the enzyme remained insensitive to carbon monoxide. The Ni-Fe active site was EPR undetectable under all conditions tested. It is concluded that the Ni-bound cyanide group is responsible for the oxygen insensitivity of the enzyme.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CYANIDE LIGAND
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 15N ENRICHMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element [NIFE]-HYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OXYGEN SENSITIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RALSTONIA EUTROPHA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Van Der Linden, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Burgdorf, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bernhard,M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bleijlevens, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Friedrich, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Albracht, S.P.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1cbX6UDOcupe_9IX2l0KQ8Wsl_3WMIs8T/view?usp=drivesdk">https://drive.google.com/file/d/1cbX6UDOcupe_9IX2l0KQ8Wsl_3WMIs8T/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8155 25.06.2025 25.06.2025 Documentos solicitados