Isolation and Cloning of 1-Aminocyclopropane-1-Carboxylate Oxidases from Carica papaya var. Eksotika I and Eksotika II (Record no. 42497)

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control field 20250625124715.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8191
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245 10 - TITLE STATEMENT
Title Isolation and Cloning of 1-Aminocyclopropane-1-Carboxylate Oxidases from Carica papaya var. Eksotika I and Eksotika II
490 0# - SERIES STATEMENT
Volume/sequential designation Acta Horticulturae, 740, p.153-162, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Sequence analysis was carried out on 1-aminocyclopropane-1-carboxylate oxidase (ACO)cDNAs from different papaya varieties available in the public databases and showed two divergent groups of ACO gene that differ significantly in the 5' and 3' untranslated regions (UTR). Two ACO cDNAs previously isolated from Eksotika I and Eksotika II papaya variety namely CAPACO1 and CP-ACO1 respectively are in the same group of ACO gene. In order to isolate another version of ACO gene from Eksotika varieties, we designed specific primers based on 5' and 3' UTR. RT-PCR method was used for Eksotika I ripe fruit while PCR screening was done on Eksotika II papaya unripe and ripe fruit cDNA libraries constructed earlier. The amplified ACO gene fragments from both Eksotika varieties had the same size of approximately 1400 bp. The putative ACO DNA fragments were then subcloned and sequenced. Homology search showed that these clones were the full-length cDNA of the other version of ACO genes. Therefore, the Eksotika I and II papaya varieties contain ACO genes from the two groups.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE (ACO)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EKSOTIKA I AND II PAPAYA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FRUIT RIPENING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element UNTRANSLATED REGIONS (UTR)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sew, Y.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lam, P.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Abu Bakar, U.K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1QOroNb8yYIEomxHJXdqLvi3nQwNmswAt/view?usp=drivesdk">https://drive.google.com/file/d/1QOroNb8yYIEomxHJXdqLvi3nQwNmswAt/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8191 25.06.2025 25.06.2025 Documentos solicitados