In Situ Extraction Strategy Affects Benzophenanthridine Alkaloid Production Fluxes in Suspension Cultures of Eschscholtzia Californica (Record no. 42499)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124715.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8193
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title In Situ Extraction Strategy Affects Benzophenanthridine Alkaloid Production Fluxes in Suspension Cultures of Eschscholtzia Californica
490 0# - SERIES STATEMENT
Volume/sequential designation Biotechnology and BioEngineering, 89(3), p.280-289, 2005
520 3# - SUMMARY, ETC.
Summary, etc. The effect of contact between cells and extractive phase on secondary metabolite production was investigated in two-phase suspension cultures of Eschscholtzia californica. A system was designed to extract benzophenanthridine alkaloids from the cell culture, without contact between XAD-7 resins and the cells: only medium was recirculated through a column packed with the extractive phase. This strategy was compared to the classic method of addition of resins directly into the cell suspension. Removal of the product directly from the medium enabled important increases in production of alkaloids, namely a 20-fold increase in sanguinarine production and a 10-fold increase in chelerythrine, with high recovery in the resin. The recirculation strategy greatly simplified the production process since the resins are easily recovered from the cell culture and enable harvest of product without termination of culture. However, due to limited flow rate, the recirculation strategy was slightly less effective than direct addition of resins into the cell suspension. In addition to enabling increased production, removal of secondary metabolites from the medium hanged metabolic flux distribution, testifying to a complex control mechanism of production.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TWO-PHASE CULTURE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ESCHSCHOLTZIA CALIFORNICA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BENZOPHENANTHRIDINE ALKALOIDS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SANGUINARINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element XAD-7 RESIN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Klvana, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Legros, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jolicoeur, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Mbhl2THbKRGnoREShN8sXmWrm7pWb8Im/view?usp=drivesdk">https://drive.google.com/file/d/1Mbhl2THbKRGnoREShN8sXmWrm7pWb8Im/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8193 25.06.2025 25.06.2025 Documentos solicitados