Structural differences between the ready and unready oxidized states of [NiFe]hydrogenases (Record no. 42504)

MARC details
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fixed length control field 02123nam a2200325Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124715.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8198
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Title Structural differences between the ready and unready oxidized states of [NiFe]hydrogenases
490 0# - SERIES STATEMENT
Volume/sequential designation J Biol Inorg Chem, 10(3), p.239-249, 2005
520 3# - SUMMARY, ETC.
Summary, etc. [NiFe]hydrogenases catalyze the reversible heterolytic cleavage of molecular hydrogen. Several oxidized, inactive states of these enzymes are known that are distinguishable by their very different activation properties. So far, the structural basis for this difference has not been understood because of lack of relevant crystallographic data. Here, we present the crystal structure of the ready Ni-B state of Desulfovibrio fructosovorans [NiFe]hydrogenase and show it to have a putative l-hydroxo Ni-Fe bridging ligand at the active site. On the other hand, a new, improved refinement procedure of the X-ray diffraction data obtained for putative unready Ni-A/Ni-SU states resulted in a more elongated electron density for the bridging ligand, suggesting that it is a diatomic species. The slow activation of the Ni-A state, compared with the rapid activation of the Ni-B state, is therefore proposed to result from the different chemical nature of the ligands in the two oxidized species. Our results along with very recent electrochemical studies suggest that the diatomic ligand could be hydro-peroxide.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element X-RAY CRYSTALLOGRAPHY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element [NIFE]HYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OXIDATIVE INACTIVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ACTIVATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Volbeda, A.
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Personal name Martin, L.
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Personal name Cavazza, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Matho, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Faber, B.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Roseboom, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Albracht, S.P.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Albracht, S.P.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rousset, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fontecilla-Camps, J.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/15m6rSpknMQbkrA5DpdjfX5YzYqH_LMkg/view?usp=drivesdk">https://drive.google.com/file/d/15m6rSpknMQbkrA5DpdjfX5YzYqH_LMkg/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8198 25.06.2025 25.06.2025 Documentos solicitados