Heterologous expression of clostridial hydrogenase in the cyanobacterium Synechococcus PCC7942 (Record no. 42525)
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| fixed length control field | 01867nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124716.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8219 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Heterologous expression of clostridial hydrogenase in the cyanobacterium Synechococcus PCC7942 |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biochimica et Biophysica Acta, 1490(3), p.269-278, 2000 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The Clostridium pasteurianum hydrogenase I has been expressed in the cyanobacterium Synechococcus PCC7942. The Shine^Dalgarno sequence of the structural gene encoding hydrogenase I from C. pasteurianum was changed to that of the cat (chloramphenicol acetyltransferase)gene. The hydrogenase gene was cloned downstream of a strong promoter, isolated from Synechococcus PCC7942, with the cat gene as a reporter gene. Expression of clostridial hydrogenase was confirmed by Western and Northern blot analyses in Synechococcus and Escherichia coli, whereas in vivo/in vitro measurements and activity staining of soluble proteins separated on non-denaturing polyacrylamide gels revealed functional expression of hydrogenase only in cyanobacterial cells. The changed Shine^Dalgarno sequence appeared to be essential for the functional expression of clostridial hydrogenase in Synechococcus, but had no influence on the expression and activity of clostridial hydrogenase expressed in E. coli. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SYNECHOCOCCUS PCC 7942 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HETEROLOGOUS GENE EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HYDROGEN PRODUCTION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Asada, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Koike, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schnackenberg, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Miyake, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Uemura, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Miyake, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1f_A7ITBN6phGBULJNEKi22psofmFXGU3/view?usp=drivesdk">https://drive.google.com/file/d/1f_A7ITBN6phGBULJNEKi22psofmFXGU3/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8219 | 25.06.2025 | 25.06.2025 | Documentos solicitados |