Intracellular localization of the hydrogenase in Desulfotomaculum orientis (Record no. 42557)

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control field 20250625124716.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8251
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245 10 - TITLE STATEMENT
Title Intracellular localization of the hydrogenase in Desulfotomaculum orientis
490 0# - SERIES STATEMENT
Volume/sequential designation FEMS MicroBiology Letters, 36(2-3), p.257, 1986
520 3# - SUMMARY, ETC.
Summary, etc. Cell extracts of Desulfotomaculum orientis, grown with H 2 plus sulfate as sole energy source, revealed hydrogenase activities between 0.3 and 2 #mol H 2 per min and nag protein when methyl viologen was used as electron acceptor. With benzyl viologen, methylene blue, FAD or FMN, lower activities were found; NAD was not reduced. The hydrogenase activity was strongly inhibited by CuC12; however, copper inhibition was not observed with whole cells, indicating that the hydrogenase is located intracellularly. After high-speed centrifugation of cell-free extracts, varying proportions, between 11 and 90
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cypionka, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dilling, W.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/13SNUdOLVkM4gt_wuFQsa5YVEBeZCW5a-/view?usp=drivesdk">https://drive.google.com/file/d/13SNUdOLVkM4gt_wuFQsa5YVEBeZCW5a-/view?usp=drivesdk</a>
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