Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis (Record no. 42588)

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control field MX-MdCICY
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control field 20250625124717.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8282
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245 10 - TITLE STATEMENT
Title Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis
490 0# - SERIES STATEMENT
Volume/sequential designation Physiological and Molecular Plant Pathology, 61(1), p.45-53, 2004
520 3# - SUMMARY, ETC.
Summary, etc. The fungal pathogen Fusarium graminearum attacks the seed spikes of barley and wheat, causing sterility, reduced seed weight and accumulation of mycotoxins. To explore infection patterns in barley and in the Arabidopsis model system, the green fluorescent protein gene (gfp)was used to transform F. graminearum. Inoculation of intact barley spikes resulted in rapid colonization of the brush hairs (ovary epithelial hairs)at the extruded seed tip within 7 h. Colonization followed a pattern of rapid basipetal growth along the pericarp epithelium (interior to the lemma and palea), accompanied by slower growth inward through the pericarp and testa. However, at 16 days after infection the aleurone and starchy endosperm remained uninfected, despite heavy colonization of the pericarp. Colonization of the outer lemma also occurred but was much slower. No increase in amylase enzyme activities was found, discounting the possibility that F. graminearum utilizes gibberellin-induced host enzymes to tap the endosperm for nutrients. The transformed Fusarium strain readily infected Arabidopsis thaliana leaves and produced copious spores within distant leaves. Results show the utility of gfp in tracing the growth of this pathogen, without misinterpretation due to contaminating fungi, and for resistance studies utilizing the Arabidopsis model system.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS THALIANA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CEREAL QUALITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUSARIUM GRAMINEARUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUSARIUM HEAD BLIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GIBBERELLA ZEAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HORDEUM VULGARE L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SCAB
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Skadsen, R.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hohn, T.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Pnnezi7htqiK40typaljCX57AQgeo23w/view?usp=drivesdk">https://drive.google.com/file/d/1Pnnezi7htqiK40typaljCX57AQgeo23w/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8282 25.06.2025 25.06.2025 Documentos solicitados