d-Aminolaevulinic acid synthesis is required for virulence of the wheat pathogen Stagonospora nodorum (Record no. 42590)
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| fixed length control field | 01968nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124717.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8284 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | d-Aminolaevulinic acid synthesis is required for virulence of the wheat pathogen Stagonospora nodorum |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | MicroBiology, 152(5), p.1533-1538, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | d-Aminolaevulinic acid (ALA)is synthesized in fungi by ALA synthase, a key enzyme in the synthesis of haem. The requirement for ALA synthase in Stagonospora nodorum to cause disease in wheat was investigated. The single gene encoding ALA synthase (Als1)was cloned and characterized. Expression analysis determined that Als1 transcription was up-regulated during germination and also towards the latter stages of the infection. The Als1 gene was further characterized by homologous gene replacement. The inactivation of Als1 resulted in strains producing severely stunted germ tubes leading quickly to death. The strains could be recovered by supplementation with 33 mM ALA. Pathogenicity assays revealed the als1 strains were essentially non-pathogenic, inferring a key role for the synthesis of ALA during in planta growth. Supplementing the strains with ALA restored growth in vitro and also pathogenicity for up to 5 days after inoculation. Further examination by inoculating the als1 strains onto wounded leaves found that pathogenicity was only partially restored, suggesting that host-derived in planta levels of ALA are not sufficient to support growth. This study has identified a key role for fungal ALA synthesis during infection and revealed its potential as an antifungal target. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Solomon, P.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jorgens, C.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Oliver, R.P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1GkcpnwaEZfQJ3Wms2oPwoG7EtM9lJizC/view?usp=drivesdk">https://drive.google.com/file/d/1GkcpnwaEZfQJ3Wms2oPwoG7EtM9lJizC/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8284 | 25.06.2025 | 25.06.2025 | Documentos solicitados |