MARC details
| 000 -LEADER |
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| fixed length control field |
02704nam a2200361Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625124719.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-8410 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Biochimica et Biophysica Acta, 1760(9), p.1323-1332, 2006 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
During the last few years, a growing number of cysteine-rich antimicrobial peptides has been isolated from plants and particularly from seeds. It has become increasingly clear that these peptides play an important role in the protection of plants against microbial infection. In this work, proteins from chilli pepper (Capsicum annuum L.)seeds were extracted in phosphate buffer, pH 5.4 and peptides purification were performed by employing ion-exchange chromatographies on DEAE, CM-Sepharose, Sephacryl S-100 and reverse phase in HPLC. Three peptide enriched fractions, namely F1, F2 and F3, were obtained after the CM-Sepharose chromatography. The F1 fraction, mainly composed of three peptides ranging from 6 to 10 kDa, was submitted to N-terminal amino acid sequencing. The closer to 10 kDa peptide showed high sequence homology to lipid transfer proteins (LTPs)previously isolated from others seeds. F1 fraction exhibited strong fungicidal activity against Candida albicans, Saccharomyces cerevisiae and schizosaccharomyces pombe and also promoted several morphological changes to C. albicans, including the formation of pseudohyphae, as revealed by scanning electron micrography. F1 fraction also reduced the glucose stimulated acidification of the medium mediated by H+-ATPase of S. cerevisiae cells in a dose-dependent manner and caused the permeabilization of yeast plasma membrane to the dye SYTOX Green, as verified by confocal laser microscopy. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LIPID TRANSFER PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MEMBRANE PERMEABILIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CAPSICUM ANNUUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT ANTIMICROBIAL PEPTIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SACCHAROMYCES CEREVISIAE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CANDIDA ALBICANS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Diz, M.S.S |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Carvalho, A.O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rodrigues, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Neves-Ferreira, A.G.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Da Cunha, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Walter Alves, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Okorokova-Façanha, A.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Okorokova-Façanha, A.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Perales, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Machado, O.L.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Gomes, V.M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1ckQFTc3lHMf-J9bfzzjo5DprBGP89Vh_/view?usp=drivesdk">https://drive.google.com/file/d/1ckQFTc3lHMf-J9bfzzjo5DprBGP89Vh_/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
