The protein kinase genes MAP3Ke1 and MAP3Ke2 are required for pollen viability in Arabidopsis thaliana (Record no. 42761)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124720.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8457
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245 10 - TITLE STATEMENT
Title The protein kinase genes MAP3Ke1 and MAP3Ke2 are required for pollen viability in Arabidopsis thaliana
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Volume/sequential designation The Plant Journal, 48(2), p.193, 2006
520 3# - SUMMARY, ETC.
Summary, etc. We have used reverse-genetic analysis to investigate the function of MAP3Ke1 and MAP3Ke2, a pair of closely related Arabidopsis thaliana genes that encode protein kinases. Plants homozygous for either map3ke1 or map3ke2 displayed no apparent mutant phenotype, whereas the double-mutant combination caused pollen lethality. Transmission of the double-mutant combination through the female gametophyte was normal. Tetrad analysis performed using the Arabidopsis quartet mutation demonstrated that the pollen-lethal phenotype segregated at meiosis with the map3ke1;map3ke2 genotype. We used transmission electron microscopy to determine that double-mutant pollen grains develop plasma embrane irregularities following pollen mitosis I. Analysis of the subcellular localization of a yellow fluorescent protein (YFP):MAP3Ke1 fusion protein using confocal microscopy and biochemical fractionation indicated that a substantial portion of the MAP3Ke1 present in Arabidopsis cells is localized to the plasma membrane. Taken together, our results suggest that MAP3Ke1 is required for the normal functioning of the plasma membrane in developing Arabidopsis pollen.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MAP3K-EPSILON
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Topical term or geographic name entry element POLLEN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASMA MEMBRANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REVERSE GENETICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chaiwongsar, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Otegui, M.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jester, P.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Monson, S.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Krysan, P.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1_CTer9s_I1ZcG37bWW1miNdNVhoE4-jz/view?usp=drivesdk">https://drive.google.com/file/d/1_CTer9s_I1ZcG37bWW1miNdNVhoE4-jz/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8457 25.06.2025 25.06.2025 Documentos solicitados