Biochemical and Molecular Characterization of AtPAP26, a Vacuolar Purple Acid Phosphatase Up-Regulated in Phosphate-Deprived Arabidopsis Suspension Cells and Seedlings1 (Record no. 42771)

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control field 20250625124720.0
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8467
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Title Biochemical and Molecular Characterization of AtPAP26, a Vacuolar Purple Acid Phosphatase Up-Regulated in Phosphate-Deprived Arabidopsis Suspension Cells and Seedlings1
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Volume/sequential designation Plant Physiology, 142, p.1282-1293, 2006
520 3# - SUMMARY, ETC.
Summary, etc. A vacuolar acid phosphatase (APase)that accumulates during phosphate (Pi)starvation of Arabidopsis (Arabidopsis thaliana)suspension cells was purified to homogeneity. The final preparation is a purple APase (PAP), as it exhibited a pink color in solution (Amax 5 520 nm). It exists as a 100-kD homodimer composed of 55-kD glycosylated subunits that cross-reacted with an anti-(tomato intracellular PAP)-IgG. BLAST analysis of its 23-amino acid N-terminal sequence revealed that this PAP is encoded by At5g34850 (AtPAP26; one of 29 PAP genes in Arabidopsis)and that a 30-amino acid signal peptide is cleaved from the AtPAP26 preprotein during its translocation into the vacuole. AtPAP26 displays much stronger sequence similarity to orthologs from other plants than to other Arabidopsis PAPs. AtPAP26 exhibited optimal activity at pH 5.6 and broad substrate selectivity. The 5-fold increase in APase activity that occurred in Pi-deprived cells was paralleled by a similar increase in the amount of a 55-kD anti-(tomato PAP or AtPAP26)-IgG immunoreactive polypeptide and a .30-fold reduction in intracellular free Pi concentration. Semiquantitative reverse transcription-PCR indicated that Pi-sufficient, Pi-starved, and Pi-resupplied cells contain similar amounts of AtPAP26 transcripts. Thus, transcriptional controls appear to exert little influence on AtPAP26 levels, relative to translational and/or proteolytic controls. APase activity and AtPAP26 protein levels were also up-regulated in shoots and roots of Pi-deprived Arabidopsis seedlings. We hypothesize that AtPAP26 recycles Pi from intracellular P metabolites in Pi-starved Arabidopsis. As AtPAP26 also
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Personal name Veljanovski, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vanderbel, B.
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Personal name Knowles, V.L.
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Personal name Snedden, W.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Plaxton, W.C.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1MIUC8TrHbTAPwErSZ-LRzOXreY5jcGPN/view?usp=drivesdk">https://drive.google.com/file/d/1MIUC8TrHbTAPwErSZ-LRzOXreY5jcGPN/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8467 25.06.2025 25.06.2025 Documentos solicitados