Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus (Record no. 42780)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02453nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124721.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8476 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Proteomics, 252, p.104434, 2022 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Understanding the mechanisms that endow a somatic cell with the ability to differentiate into a somatic embryo, which could result in numerous biotechnological applications, is still a challenge. The objective of this work was to identify some of the molecular and physiological mechanisms responsible for the acquisition of embryogenic competence during somatic embryogenesis in Carica papaya L. We performed a broad characterization of embryogenic (EC)and nonembryogenic calli (NEC)of using global and mitochondrial proteomic approaches, histomorphology, histochemistry, respiratory activity, and endogenous hormonal and hydrogen peroxide (H2O2)contents. EC and NEC presented remarkable differences in anatomical and histochemical characteristics, with EC showing a higher reactivity for the presence of proteins and neutral polysaccharides. Our results demonstrate that mitochondrial metabolism affects the embryogenic competence of C. papaya callus. The EC presented higher participation of alternative oxidase (AOX)enzymes, higher total cell respiration and presented a stronger accumulation of mitochondrial stress response proteins. Differential accumulation of auxin-responsive Gretchen Hagen 3 (GH3)family proteins in EC was related to a decrease in the content of free 2,4-dichlorophenoxyacetic acid (2,4-D). EC also showed higher endogenous H2O2 contents. H2O2 is a promising molecule for further investigation in differentiation protocols for C. papaya somatic embryos. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HORMONES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LABEL-FREE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MITOCHONDRIAL PROTEOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vale, E. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Santana, D. B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reis, R. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sousa, K. R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Souza Filho, G. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Oliveira, J. G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Silveira, V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/190bR6GiGSoJxqS01MbOaXcu3FJvWuM50/view?usp=drivesdk">https://drive.google.com/file/d/190bR6GiGSoJxqS01MbOaXcu3FJvWuM50/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8476 | 25.06.2025 | 25.06.2025 | Documentos solicitados |