Detection of Tomato Yellow leaf Curl Thailand Virus by PCR Without DNA Extraction (Record no. 42883)

MARC details
000 -LEADER
fixed length control field 02054nam a2200265Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124723.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8579
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Detection of Tomato Yellow leaf Curl Thailand Virus by PCR Without DNA Extraction
490 0# - SERIES STATEMENT
Volume/sequential designation Molecular Biotechnology, 31(3), p.233-238, 2005
520 3# - SUMMARY, ETC.
Summary, etc. We report the simple and rapid method detection of tomato yellow leaf curl Thailand virus (TYLCTHV)based on the direct capture of virus partic1es to the surface of a polymerase chain reaction (PCR)tube. This method allowed PCR without the time-consuming pracedures of DNA extraction fram infected plant tissue. A small amount of tomato was ground in extraction Buffer to release viruses fram plant tissues. The sconstituents of the plant extract that might inhibit PCR activity were discarded by washing the tube with PBST buffer before adding the PCR mixture to the tube. This method was used for detection of TYLCTHV -withplant sap solution diluted up to 1:20 ,000 and was more sensitive than an enzyme-linked.immunosorbent assay (ELISA)method. In addition, this method can be used for detection of TYLCTHV in viruliferous whiteflies. The PCR tubes with captured TYLCTHV could be used for PCR, after storage at 4°C for 4 wk. The method presented here was used for detection of begomoviruses in cucurbit and pepper. In addition, this method was effectively used to detect papaya ringspot virus in papaya and zucchini yellow mosaic virus in cucumber by reverse transcriptase (RT)-PCR.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TOMATO YELLOW LEAF CTIRL THAILAND VIRUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GEM.INIVIRUSES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BEGOMOVIRUSES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RT-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA EXTRACTION.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name leamkhang, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Riangwong, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chatchawankanphanich, O.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/15MIV--jdM3m-P9yOx-7KKacc0CBt94wv/view?usp=drivesdk">https://drive.google.com/file/d/15MIV--jdM3m-P9yOx-7KKacc0CBt94wv/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8579 25.06.2025 25.06.2025 Documentos solicitados