Comparison of two assay procedures for lignin peroxidase (Record no. 42933)

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control field 20250625124724.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8629
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Title Comparison of two assay procedures for lignin peroxidase
490 0# - SERIES STATEMENT
Volume/sequential designation Enzyme and Microbial Technology, 28(7-8), p.602-605, 2001
520 3# - SUMMARY, ETC.
Summary, etc. The most widely accepted assay for detecting lignin peroxidase, based on the oxidation of veratryl alcohol to veratraldehyde, suffers from some drawbacks. At 310 nm, the wavelength at which the assay is performed, some other materials like lignins, quinonic compounds and aromatics also exhibit strong absorbance thus interfering with the estimation when present in the media. The present study reports the lignin peroxidase production by some white rot fungi under different nutritional conditions. The veratryl alcohol oxidation assay procedure for lignin peroxidase has been compared with another method based on the oxidation of the dye azure B involving absorbance measurements in the visible range. The latter method proved to be much more advantageous over the veratryl alcohol oxidation method, in media supplemented with malt extract, lignin preparations and agricultural residues. The enzyme production by veratryl alcohol assay could be detected only in mineral salts broth. By the azure B assay the enzyme activity was detected in all the media tested. The supplements gave varied response in different media. Veratryl alcohol enhanced the enzyme production in malt extract broth and mineral salts malt extract broth. Among the lignin preparations Indulin AT increased the lignin peroxidase titres from 2 to 20 fold in different fungi. Similarly, wheat straw supplemented in mineral salts broth and malt extract broth, separately, strongly stimulated the lignin peroxidase production. The above studies revealed that azure B assay may act as a substitute or equivalent method.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AZURE B
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIGNIN PEROXIDASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VERATRYL ALCOHOL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element WHITE ROT FUNGI
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arora, D.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gill, P.K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xJgKC6oAfGSeVvjPhNYLF7egGaVGnpL0/view?usp=drivesdk">https://drive.google.com/file/d/1xJgKC6oAfGSeVvjPhNYLF7egGaVGnpL0/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8629 25.06.2025 25.06.2025 Documentos solicitados