Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development (Record no. 42945)

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control field MX-MdCICY
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control field 20250625124724.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8642
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Title Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development
490 0# - SERIES STATEMENT
Volume/sequential designation The Plant Journal, 9(5), p.745-753, 1996
520 3# - SUMMARY, ETC.
Summary, etc. Using a highly synchronous in vitro tuberization system, in combination with an amplified restriction fragment polymorphism (AFLpt)-derived technique for RNA fingerprinting (cDNA-AFLPt), transcriptional changes at and around the time point of potato tuberization have been analyzed. The targeted expression analysis of a specific transcript coding for the major potato storage protein, patatin and a second transcript, coding for ADP-glucose pyrophosphorylase, a key gene in the starch biosynthetic pathway is described. This paper confirms that kinetics of expression revealed by cDNA-AFLP analysis are comparable to those found in Northern analysis. Furthermore, this paper reports the isolation and analysis of two tuberspecific transcript-derived fragments (TDFs)coding for the lipoxygenase enzyme, which are differentially induced around the time point of tuber formation. Analysis of the two Iox TDFs demonstrates that it is possible to dissect the expression modalities of individual transcripts, not independently detectable by Northern analysis. Finally, it is shown that using cDNA-AFLP, rapid and simple verification of band identity may be achieved. The results indicate that cDNA-AFLP is a broadly applicable technology for identifying developmentally regulated genes.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bachem, C.W.B.
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Personal name van der Hoeven, R.S.
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Personal name de Bruijn, S.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vreugdenhil, D.
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Personal name Zabeau, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Visser, R.G.F.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1NCcSS8Q4A2y8ujLyz-SJ7W0tSweim4Zo/view?usp=drivesdk">https://drive.google.com/file/d/1NCcSS8Q4A2y8ujLyz-SJ7W0tSweim4Zo/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8642 25.06.2025 25.06.2025 Documentos solicitados