Observations of Mitochondria and Mitochondrial Nuclei by Double Staining with Rhodamine-123 and DAPI in Synchronous Cultures of Catharanthus roseus (Record no. 42946)

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fixed length control field 01684nam a2200241Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124724.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8643
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245 10 - TITLE STATEMENT
Title Observations of Mitochondria and Mitochondrial Nuclei by Double Staining with Rhodamine-123 and DAPI in Synchronous Cultures of Catharanthus roseus
490 0# - SERIES STATEMENT
Volume/sequential designation The Botanical Magazine, Tokyo, 105(3), p.405-411, 1992
520 3# - SUMMARY, ETC.
Summary, etc. Mitochondria in cells of Catharanthus roseus (L.)G. Don in synchronous cell division cultures were observed by double staining using fluorescence microscopy. The cells were stained with 4'-6-diamidino-2-phenylindole (DAPI)first and subsequently stained with rhodamine 123 (r-123). Immediately after staining with r-123, yellowishgreen, elongated and moving mitochondria were observed upon excitation at 485 nm. When the excitation filters were replaced by a UV filter (360 n ), 1 to 7 mitochondrial nucleoids were visible in each mitochondrion in the same field. Changes in the lengths of mitochondria during the cell cycle obtained from the observations under fluorescence microscopy by this staining method suggest the occurrence of multiplication of mitochondria concurrent with the cell cycle of C. roseus.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CATHARANTHUS ROSEUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL CYCLE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DAPI
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RHODAMINE 123
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DOUBLE STAINING OF MITOCHONDRIA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sakiko Hirose
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Atsushi Komamine
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1j8mYjeI9u3XcFS_nhhR1rSb0z2WGd9N6/view?usp=drivesdk">https://drive.google.com/file/d/1j8mYjeI9u3XcFS_nhhR1rSb0z2WGd9N6/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8643 25.06.2025 25.06.2025 Documentos solicitados