Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco (Record no. 42959)

MARC details
000 -LEADER
fixed length control field 02204nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124724.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8656
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Journal, 50(5), p.917-925, 2007
520 3# - SUMMARY, ETC.
Summary, etc. A two-component expression system was developed to achieve tightly regulated expression of transgenes in plants. One component functioned as an expression module whereas the other functioned as a regulatory module. The expression module comprised a highly expressing TATA-dependent seed-specific promoter in which the TATA motif in the core promoter was mutated to TGTA. The regulatory module expressed a mutated general transcription factor TBPm3 that recognized TGTA and initiated transcription. Vectors were designed using component one alone or in combination with component two, and were transformed into tobacco. The TGTA mutation in the TATA-box completely inactivated the promoter, making component one non-functional. This non-functional module became transcriptionally active in the presence of the component two that expressed TBPm3. The reporter gene gusA was expressed from the TGTA-containing chimeric legumin promoter, in a tightly seed-specific manner, in transgenic tobacco plants in the presence of TBPm3 that was expressed from a constitutive promoter. The results show that the TGTA and TBPm3 combination can be used to achieve high-level tissue-specific expression of TATA-dependent promoters.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LEGUMIN PROMOTER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TATA BOX
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TATA BINDING PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TBP MUTANT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EXPRESSION MODULE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TIGHTLY REGULATED PROMOTER
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chaturvedi, C.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lodhi, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ansari, S.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tiwari, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Srivastava, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sawant, S.V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tuli, R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1a0HXXdn15XH2jtY0X_uYTfG9fiP3EIuh/view?usp=drivesdk">https://drive.google.com/file/d/1a0HXXdn15XH2jtY0X_uYTfG9fiP3EIuh/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8656 25.06.2025 25.06.2025 Documentos solicitados