Disulfide Bridges in the Mesophilic Triosephosphate Isomerase from Giardia lamblia Are Related to Oligomerization and Activity (Record no. 43022)
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| 000 -LEADER | |
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| fixed length control field | 02654nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124725.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8720 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Disulfide Bridges in the Mesophilic Triosephosphate Isomerase from Giardia lamblia Are Related to Oligomerization and Activity |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | J. Mol. Biol., 365(3), p.752-763, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Triosephosphate isomerase from the mesophile Giardia lamblia (GlTIM)is the only known TIM with natural disulfide bridges. We previously found that oxidized and reduced thiol states of GlTIM are involved in the interconversion between native dimers and higher oligomeric species, and in the regulation of enzymatic activity. Here, we found that trophozoites and cysts have different oligomeric species of GlTIM and complexes of GlTIM with other proteins. Our data indicate that the internal milieu of G. lamblia is favorable for the formation of disulfide bonds. Enzyme mutants of the three most solvent exposed Cys of GlTIM (C202A, C222A, and C228A)were prepared to ascertain their contribution to oligomerization and activity. The data show that the establishment of a disulfide bridge between two C202 of two dimeric GlTIMs accounts for multimerization. In addition, we found that the establishment of an intramonomeric disulfide bond between C222 and C228 abolishes catalysis. Multimerization and inactivation are both reversed by reducing conditions. The 3D structure of the C202A GlTIMwas solved at 2.1 Å resolution, showing that the environment of the C202 is prone to hydrophobic interactions. Molecular dynamics of an in silico model of GlTIM when the intramonomeric disulfide bond is formed, showed that S216 is displaced 4.6 Å from its original position, causing loss of hydrogen bonds with residues of the active-site loop. This suggests that this change perturb the conformational state that aligns the catalytic center with the substrate, inducing enzyme inactivation. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GIARDIA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRIOSEPHOSPHATE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DISULFIDE BONDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GLYCOLYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DINAMIC MOLECULAR |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reyes-Vivas, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Diaz, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Peon, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mendoza-Hernandez, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hernandez-Alcantara, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De La Mora-De La Mora, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Enriquez-Flores, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Enriquez-Flores, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lopez-Velazquez, G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1sQnl0ZZsV-JamvNvnXFz_SVxpKzIwEZ7/view?usp=drivesdk">https://drive.google.com/file/d/1sQnl0ZZsV-JamvNvnXFz_SVxpKzIwEZ7/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8720 | 25.06.2025 | 25.06.2025 | Documentos solicitados |