Accumulation of O2-tolerant phenotypes in H2-producing strains of Chlamydomonas reinhardtii by sequential applications of chemical mutagenesis and selection (Record no. 43049)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124726.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8748
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245 10 - TITLE STATEMENT
Title Accumulation of O2-tolerant phenotypes in H2-producing strains of Chlamydomonas reinhardtii by sequential applications of chemical mutagenesis and selection
490 0# - SERIES STATEMENT
Volume/sequential designation International Journal of Hydrogen Energy, 27, p.1421-1430, 2002
520 3# - SUMMARY, ETC.
Summary, etc. The photoproduction of hydrogen by anaerobically induced algae is catalyzed by a bidirectional hydrogenase that is rapidly inactivated by oxygen. We isolated two generations of Chlamydomonas reinhardtii strains with H2-evolving activities of up to 10 times the O2-tolerance seen in the wild-type (WT). These isolates were generated by two sequential selections, consisting of random chemical mutagenesis, enrichment for H2-metabolism clones following exposure to increasing amounts of O2, and screening using a chemochromic sensor. The selected strains were characterized by two types of assays and classi4ed as those that (a)can evolve H2 following exposure to O2 concentrations that inactive the WT strain and (b)in addition, are able to quickly reactivate H2-production activity once O2 is removed. These results suggest that O2-tolerance can be increased by successive rounds of mutagenesis, selection, and screening, demonstrating that the WT phenotype can be improved by genetic means. Other results show that the hydrogenase is less sensitive to O2 when it is actively catalyzing H2 evolution. Published by Elsevier Science Ltd on behalf of the International Association for Hydrogen Energy.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GREEN ALGAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLAMYDOMONAS REINHARDTII
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element H2 PHOTOPRODUCTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element O2 TOLERANCE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Flynn, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ghirardi, M.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seibert, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1KCFbHi-3Pk3WWkcFEPNXR225hR1xd7nn/view?usp=drivesdk">https://drive.google.com/file/d/1KCFbHi-3Pk3WWkcFEPNXR225hR1xd7nn/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8748 25.06.2025 25.06.2025 Documentos solicitados