Dilution methods to deprive Chlamydomonas reinhardtii cultures of sulfur for subsequent hydrogen photoproduction (Record no. 43050)

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control field 20250625124726.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8749
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245 10 - TITLE STATEMENT
Title Dilution methods to deprive Chlamydomonas reinhardtii cultures of sulfur for subsequent hydrogen photoproduction
490 0# - SERIES STATEMENT
Volume/sequential designation International Journal of Hydrogen Energy, 27, p.1245-1249, 2002
520 3# - SUMMARY, ETC.
Summary, etc. Sulfur deprivation of Chlamydomonas reinhardtii cultures gradually inactivates photosynthetic O2 evolution and leads to the establishment of anaerobiosis in the medium. Sulfur-deprived algal cultures kept under anaerobic conditions will then produce H2 gas for 3-5 days under continuous illumination. Currently, sulfur deprivation is achieved by mechanical centrifugation of cultures grown in sulfur-replete medium, followed by extensive and costly washing. The cells are 8nally resuspended in sulfur-free medium. The current study investigates two procedures to deprive algal cultures of sulfur that eliminate the centrifugation step. These procedures involve sulfur deprivation by dilution of sulfur-replete cultures into either sulfur-limited medium or sulfur-free medium. We demonstrate that e:cient H2 photoproduction can be achieved on a timely basis using either procedure. However, the dilution of sulfate-replete algal cultures 1:10 v=v into sulfur-free medium is the most appropriate procedure. These observations serve as the basis for developing an algal H2-production system that is cheaper, less time-consuming, and less amenable to contamination with other microorganisms than systems employing centrifugation for sulfur deprivation.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOTOBIOLOGICAL HYDROGEN PRODUCTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SULFUR DEPRIVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GREEN ALGAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOTOBIOREACTORS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Laurinavichene, T.V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tolstygina, I.V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Galiulina, R.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ghirardi, M.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seibert, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tsygankov, A.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1mwEtyxZQJS4GKBrN6UgEJi_FSQp_DpA7/view?usp=drivesdk">https://drive.google.com/file/d/1mwEtyxZQJS4GKBrN6UgEJi_FSQp_DpA7/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8749 25.06.2025 25.06.2025 Documentos solicitados