Constitutive hydrolytic enzymes are associated with polygenic resistance of tomato to Alternaria solani and may function as an elicitor release mechanism (Record no. 43107)

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fixed length control field 02773nam a2200253Ia 4500
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control field MX-MdCICY
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control field 20250625124727.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8806
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245 10 - TITLE STATEMENT
Title Constitutive hydrolytic enzymes are associated with polygenic resistance of tomato to Alternaria solani and may function as an elicitor release mechanism
490 0# - SERIES STATEMENT
Volume/sequential designation Physiological and Molecular Plant Pathology, 57, p.211-220, 2000
520 3# - SUMMARY, ETC.
Summary, etc. Foliar resistance to early blight disease of tomato, caused by the necrotrophic fungus Alternaria solani, is inherited in a complex quantitative manner. Our previous studies revealed that three moderately-resistant tomato breeding lines with di_erent sources of early blight resistance, all possessed higher constitutive and more rapid accumulation of PR proteins, including speciRc antifungal isozymes of chitinase and b-1,3-glucanase than susceptible genotypes. In the present study, additional early blight resistant tomato breeding lines and susceptible genotypes were investigated for their constitutive levels of PR proteins. All resistant lines possessed higher constitutive levels of chitinase and b-1,3-glucanase than susceptible genotypes supporting earlier reported Rndings. Constitutive and pathogen-induced expression of PR genes during early stages of the interaction was found to be much higher in the highly resistant breeding line NC 24-E than a susceptible line. In vitro elicitor release experiments were performed with puriRed A. solani cell walls treated with constitutive total enzyme preparations isolated from resistant and susceptible plants. Enzyme preparations from resistant and susceptible genotypes di_ered in their ability to produce hypersensitive response (HR)elicitors from fungal cell walls: the enzyme preparations from resistant breeding lines released HR elicitors, while enzyme reparations from susceptible lines did not. Additionally, experiments with a partially puriRed preparation of basic tomato chitinases demonstrated that these enzymes were able to release HR elicitors from germinating spores of A. solani, but not mature, intact cell walls. The possibility that constitutively produced hydrolytic enzymes may act as an elicitor- releasing mechanism in resistance to early blight of tomato is discusse
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALTERNARIA SOLANI
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LYCOPERSICON ESCULENTUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PATHOGENESIS-RELATED PROTEINS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name LAWRENCE, C.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name SINGH, N.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name RIU, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name GARDNER, R.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name TUZUN, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/13qCRIgnyIlJhToB9Vy4l5C6Rhb5maWVB/view?usp=drivesdk">https://drive.google.com/file/d/13qCRIgnyIlJhToB9Vy4l5C6Rhb5maWVB/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8806 25.06.2025 25.06.2025 Documentos solicitados