Successful Gene Tagging in Lettuce Using the Tnt1 Retrotransposon from Tobacco (Record no. 43123)
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| fixed length control field | 02076nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124728.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8843 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Successful Gene Tagging in Lettuce Using the Tnt1 Retrotransposon from Tobacco |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Physiology, 144, p.18-31, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The tobacco (Nicotiana tabacum)element Tnt1 is one of the few identified active retrotransposons in plants. These elements possess unique properties that make them ideal genetic tools for gene tagging. Here, we demonstrate the feasibility of gene tagging using the retrotransposon Tnt1 in lettuce (Lactuca sativa), which is the largest genome tested for retrotransposon mutagenesis so far. Of 10 different transgenic bushes carrying a complete Tnt1 containing T-DNA, eight contained multiple transposed copies of Tnt1. The number of transposed copies of the element per plant was particularly high, the smallest number being 28. Tnt1 transposition in lettuce can be induced by a very simple in vitro culture protocol. Tnt1 insertions were stable in the progeny of the primary transformants and could be segregated genetically. Characterization of the sequences flanking some insertion sites revealed that Tnt1 often inserted into genes. The progeny of some primary transformants showed phenotypic alterations due to recessive mutations. One of these mutations was due to Tnt1 insertion in the gibberellin 3b-hydroxylase gene. Taken together, these results indicate that Tnt1 is a powerful tool for insertion mutagenesis especially in plants with a large genome. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mazier, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Botton, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Flamain, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bouchet, J.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Courtial, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chupeau, M.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chupeau, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chupeau, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lucas, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1TzfuNIHcshz9qvR8mstSP8SDMgo0dQaX/view?usp=drivesdk">https://drive.google.com/file/d/1TzfuNIHcshz9qvR8mstSP8SDMgo0dQaX/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8843 | 25.06.2025 | 25.06.2025 | Documentos solicitados |