Immobilization of glucosidase onto silica-based, amino functionalized beads for enzymatic hydrolysis of urinary phenol prior to liquid chromatographic analysis (Record no. 43131)
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| 000 -LEADER | |
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| fixed length control field | 01810nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124728.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8869 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Immobilization of glucosidase onto silica-based, amino functionalized beads for enzymatic hydrolysis of urinary phenol prior to liquid chromatographic analysis |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytica Chimica Acta, 292(1-2), p.23-30, 1994 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | An amino-functionalized silica commercial liquid chromatography packing material was used instead of controlled- pore glass as the support to react with glutaraldehyde and then to directly immobilize the /3-glucosidase. After enzymatic hydrolysis, the urinary phenol was separated by reversed-phase liquid chromatography and monitored by an electrochemical detector at 0.85 V (vs. Ag/AgCl). Factors that affect the immobilization and hydrolysis were investigated. The proposed method provides a simpler procedure with a less expensive material for glucosidase immobilization, and the electrochemical detection avoids the interference from other urinary species and hence simplifies the chromatogram obtained. The detection limit was 0.2 ng with a 28 ~1 injection. The relative standard deviations for 1.2 ng and 2.0 ng levels of phenols were 1.16 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENZYMATIC METHODS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LIQUID CHROMATOGRAPHY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PACKING MATERIALS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GLUCOSIDASE IMMOBILIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHENOL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | URINARY PHENOL |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jen, J.F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zen, J.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cheng, F.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, G.Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1ItNie1pJVIj6OGN3WdRX0UiARS5_S5iE/view?usp=drivesdk">https://drive.google.com/file/d/1ItNie1pJVIj6OGN3WdRX0UiARS5_S5iE/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8869 | 25.06.2025 | 25.06.2025 | Documentos solicitados |