Emulsion PCR-based method to detect Y chromosome microdeletions (Record no. 43140)
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| 000 -LEADER | |
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| fixed length control field | 01770nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124728.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8878 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Emulsion PCR-based method to detect Y chromosome microdeletions |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 367(2), p.173-178, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Deletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs)distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EMULSIONS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MULTIPLEX PCR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Y CHROMOSOME MICRODELETIONS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ge, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bai, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yu, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lu, Z. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1lRYB7RBZhI5cKmK3S5wX4zmf1f2llgJu/view?usp=drivesdk">https://drive.google.com/file/d/1lRYB7RBZhI5cKmK3S5wX4zmf1f2llgJu/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8878 | 25.06.2025 | 25.06.2025 | Documentos solicitados |