MARC details
| 000 -LEADER |
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| fixed length control field |
02261nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625124729.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-8937 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Plant DNA from alcohol-preserved samples |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Molecular Biology Reporter, 14(3), p.261-265, 1996 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A process to isolate DNA from alcohol-preserved plant tissue is described. Key features are simple, inexpensive sample preservation, fast tissue disruption, and no organic solvents. Tissue preservation and homogenization are two rate-limiting steps and are major nuisances in the isolation of plant DNA. Fresh samples require hand grinding, which is problematic due to large tissue volumes and sample numbers, or when plants are grown in a remote location. Freezing requires equipment, complicates shipping, and the grinding is tedious. Freeze drying (Murray and Thompson, 1980)requires expensive equipment on location, although more recently, Tai and Tanksley (1990)demonstrated that a food dehydrator can be substituted. Regardless of how tissue is dried, grinding of dry samples remains tedious and messy. The reduction in DNA quantities needed for various PCR-based techniques has made all of the above somewhat easier, but situations remain where one needs DNA in the range of tens of [mu]g to mg. We avoid freezing or drying by pickling the plant tissue in reagent alcohol. Then we grind in a buffer containing hexylene-glycol for the isolation of nuclei, which makes nuclei tough enough to allow tissue disruption with a PolytronTM homogenizer. The preparation of a crude nuclear pellet achieves sufficient purification that purification extraction of the organic phase is generally unnecessary. The resulting DNA is clean enough for Southern blots and PCR-based techniques, though not necessarily of high enough quality for genomic cloning. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NUCLEI |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RAPD |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RFLP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SPAR |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Murray, M.G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Pitas, J.W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/122ne3QPU-TAFbBYhMpCFKrU1N9n4Fhq6/view?usp=drivesdk">https://drive.google.com/file/d/122ne3QPU-TAFbBYhMpCFKrU1N9n4Fhq6/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
