Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups (Record no. 43236)
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| fixed length control field | 02038nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124730.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-8974 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | International Journal of Systematic and Evolutionary MicroBiology, 57, p.1855-1867, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Phytoplasmas are cell wall-less bacteria that cause numerous plant diseases. As no phytoplasma has been cultured in cell-free medium, phytoplasmas cannot be differentiated and classified by the traditional methods which are applied to culturable prokaryotes. Over the past decade, the establishment of a phytoplasma classification scheme based on 16S rRNA restriction fragment length polymorphism (RFLP)patterns has enabled the accurate and reliable identification and classification of a wide range of phytoplasmas. In the present study, we expanded this classification scheme through the use of computer-simulated RFLP analysis, achieving rapid differentiation and classification of phytoplasmas. Over 800 publicly available phytoplasma 16S rRNA gene sequences were aligned using the CLUSTAL_X program and the aligned 1.25 kb fragments were exported to pDRAW32 software for in silico restriction digestion and virtual gel plotting. Based on distinctive virtual RFLP patterns and calculated similarity coefficients, phytoplasma strains were classified into 28 groups. The results included the classification of hundreds of previously unclassified phytoplasmas and the delineation of 10 new phytoplasma groups representing three recently described and seven novel putative 'Candidatus Phytoplasma' taxa. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wei, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Davis, R.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, I.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1hx9AUb1Iu_Ji0zgPB8bQFEVcEaWSDr4-/view?usp=drivesdk">https://drive.google.com/file/d/1hx9AUb1Iu_Ji0zgPB8bQFEVcEaWSDr4-/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-8974 | 25.06.2025 | 25.06.2025 | Documentos solicitados |