Methylene Blue Stainability of Root-lSp Protoplasts as an Indicator of Aluminum Tolerance in a Wide Range of Plant Species, Cultivars and Lines (Record no. 43282)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124731.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9022
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245 10 - TITLE STATEMENT
Title Methylene Blue Stainability of Root-lSp Protoplasts as an Indicator of Aluminum Tolerance in a Wide Range of Plant Species, Cultivars and Lines
490 0# - SERIES STATEMENT
Volume/sequential designation Soil Sci. Plant Nutr., 51(7), p.991-998, 2005
520 3# - SUMMARY, ETC.
Summary, etc. We previously developed a new simple technique of methylene blue (MB)staining for the discrimination of aluminum (Al)-tolerant protoplasts from 4 plant species (rice, oats, maize and pea). The objectives of the present study were to conflrm the applicability of this technique to a wider range of plant species, cultivars and lines, and to identify a common strategy for the early stage of Al tolerance. A total of 10 plant species, two rachiaria spp., two Oryza spp., buckwheat, maize, pea, triticale, wheat and barley, corresponding to 18 different plant samples (species, cultivars, and lines), were used. Al tolerance (relative net root elongation of the longest root), which was screened at 20 pM AlC13 in 0.2 mM CaC12 (pH 4.9)for 24 h, ranged widely from 10 to 88. Among cultivars and lines within the same species, Al accumulation in the root tip portion was higher in Al-sensitive plants, corresponding to more severe permeabilization of the plasma membranes (PM). Protoplasts isolated mainly from the epidermis, and outer and central parts of the cortex were stained to different degrees by MB, and the blue color was observed both on the surface and inside the protopladlts. Color pictures obtained after staining for 3 min with 0.1
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALUMINUM TOLERANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METHYLENE BLUE STAINABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASMA MEMBRANE NEGATIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLASMA MEMBRANE PERMEABILITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOPLAST
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wagatsuma, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hossain Khan, M.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rao, I.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wenzl, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tawaraya, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yamamoto, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kawamura, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kawamura, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ishikawa, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1oG4E5suwCM-7vcrC6rk9xsYbvL8xY1tr/view?usp=drivesdk">https://drive.google.com/file/d/1oG4E5suwCM-7vcrC6rk9xsYbvL8xY1tr/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9022 25.06.2025 25.06.2025 Documentos solicitados