Agrobacterium-mediated transformation of Sclerotinia sclerotiorum (Record no. 43307)

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control field MX-MdCICY
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control field 20250625124731.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9048
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Title Agrobacterium-mediated transformation of Sclerotinia sclerotiorum
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Volume/sequential designation Journal of MicroBiological Methods, 65(1), p.220-207, 2006
520 3# - SUMMARY, ETC.
Summary, etc. Ascospores from the phytopathogenic fungus Sclerotinia sclerotiorum were transformed to hygromycin B resistance by co-cultivation with Agrobacterium tumefaciens. Transformed spores germinated and grew on PDA supplemented with 100 ug/ml hygromycin B. The presence of mitotically stable hph gene integration at random sites in the genome was confirmed by PCR and Southern blot analysis. A transformation frequency of 8 × 10- 5 was achieved in five separate experiments. This study is the first report of success co-cultivating A. tumefaciens with S. sclerotiorum. This report of a reproducible Agrobacterium-mediated transformation method should allow the development of T-DNA tagging as a system for insertional mutagenesis in S. sclerotiorum and provide a simple and reliable method for genetic manipulation.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Welda, R. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Colin, C. E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ridgwaya, H. J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1lMPEK8erbud-SSE3qm_hK2_EguHy1yDp/view?usp=drivesdk">https://drive.google.com/file/d/1lMPEK8erbud-SSE3qm_hK2_EguHy1yDp/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9048 25.06.2025 25.06.2025 Documentos solicitados