Potential for introducing cold tolerance into papaya by transformation with C-repeat binding factor (CBF)genes (Record no. 43392)

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control field MX-MdCICY
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control field 20250625124733.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9134
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245 10 - TITLE STATEMENT
Title Potential for introducing cold tolerance into papaya by transformation with C-repeat binding factor (CBF)genes
490 0# - SERIES STATEMENT
Volume/sequential designation In Vitro Cellular & Developmental Biology-Plant, 43(3), p.195-2002, 2007
520 3# - SUMMARY, ETC.
Summary, etc. production is affected by low temperatures that occur periodically in the subtropics.The C-repeat binding factor (CBF)gene family is known to induce the cold acclimation pathway in Arabidopsis thaliana. Embryogenic papaya cultures were induced from hypocotyls of "Sunrise Solo" zygotic embryos on semisolid induction medium. The CBF 1/CBF 3 genes along with the neomycin phosphotransferase (NPT II)gene were placed under the control of the CaMV 35 S promoter and introduced into a binary vector pGA 643. Embryogenic cultures were transformed with Agrobacterium strain GV 3101 harboring pGA 643. After selection of transformed embryogenic cultures for resistance to 300 mg l-1 kanamycin, somatic embryo development was initiated and transgenic plants were regenerated. The presence of the CBF transgenes in regenerated plants was confirmed by Southern blot hybridization. The papaya and the related cold-tolerant Vasconcella genomes were probed for the presence of cold inducible sequences using polymerase chain reaction (PCR). Possible cold inducible sequences were present in the Vasconcella genome but were absent in the Carica genome.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM TUMEFACIENS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARICA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENETIC TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYO
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dhekney, S. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Litz, R. E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Moraga Amador, D. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yadav, A. K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1gPfMzC9MxUlA-GStCdx4HKASIqilTe9V/view?usp=drivesdk">https://drive.google.com/file/d/1gPfMzC9MxUlA-GStCdx4HKASIqilTe9V/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9134 25.06.2025 25.06.2025 Documentos solicitados