A method for extracting intact chloroplast and cytoplasmic ribosomal RNA from leaves. (Record no. 43414)

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control field MX-MdCICY
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control field 20250625124733.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9157
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245 10 - TITLE STATEMENT
Title A method for extracting intact chloroplast and cytoplasmic ribosomal RNA from leaves.
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Volume/sequential designation BioChemical and biophysical Research Communications, 51(4), p.993-999, 1973
520 3# - SUMMARY, ETC.
Summary, etc. A method is described for extracting intact chloroplast and cytoplasmic ribosomal RNA from leaves of two higher plant species. Sodium dodecyl sulfate (1percent)and 25 mM magnesium ions are required to inhibit ribonuclease action during RNA purification by phenol deproteinization. The ethanol-precipitated RNA product, including 23s chloroplast ribosomal RNA, is completely stable during electrophoresis in the absence of magnesium ions, even in the presence of EDTA. The -i-n vivo mole fraction of chloroplast ribosomes relative to cytoplasmic ribosomes is estimated. Bentonite is shown to cause preferential losses of chloroplast RNA during extraction.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bourque, D. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hagiladi, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Naylor, A. W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1VkmoJxFf3OKxvLEdEzHtC9yQk_04fSua/view?usp=drivesdk">https://drive.google.com/file/d/1VkmoJxFf3OKxvLEdEzHtC9yQk_04fSua/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9157 25.06.2025 25.06.2025 Documentos solicitados