Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers (Record no. 43439)
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| fixed length control field | 01826nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124734.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9183 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Zoo Biology, 451, p.1013-1017, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The ability to rapidly and reliably determine the sex of birds is very important for successful captive-bird breeding programs, as well as for field research. Visual inspection of adult birds is sufficient for sexually dimorphic species, but nestlings and monomorphic species are difficult, if not impossible, to sex by sight only. A method for rapid extraction of gDNA from blood, shell-membrane blood vessels, and fully grown feathers, using Chelex, and the PCR conditions for determination of sex-specific bands in 47 species (39 genera, 21 families, and 10 orders)are described. The PCR primers used amplify a length of DNA spanning an intron in the CHD-1 gene, which is present on both the W and Z chromosomes. The intron differs in size between the two sex chromosomes, resulting in PCR products that separate into two bands for females and a single band for males in most avian species (except ratites). Because this simple technique uses Chelex, a rapid gDNA isolation protocol, and sets of PCR primers independent of restriction enzyme digestion, birds can be accurately sexed within 5 hr of sample collection. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jensen, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pernasetti, F. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Durrant, B. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/18f1w5vNs-iqRDDRj7Rd2Ha5Tkn45_6QV/view?usp=drivesdk">https://drive.google.com/file/d/18f1w5vNs-iqRDDRj7Rd2Ha5Tkn45_6QV/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9183 | 25.06.2025 | 25.06.2025 | Documentos solicitados |